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KMID : 1094720140190050790
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 5 p.790 ~ p.797
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A dps promoter based expression system for improved solubility of expressed proteins in Escherichia coli
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Sethia Piyush P.
Rao K. Krishnamurthy
Noronha Santosh B.
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Abstract
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Escherichia coli is widely used for recombinant protein production due to its well established genetic manipulation techniques and cost effectiveness of the associated production processes. Soluble expression of heterologous recombinant proteins constitutes a major problem in the deployment of bacterial expression systems. We have developed a dps promoter based expression system in E. coli for improved solubility of expressed proteins. The resulting expression system was found to be superior to the IPTG inducible T7 promoter based pET expression system for production of soluble β-galactosidase, tdTomato, and mCherry. The dps promoter based expression system was shown to be functional in most commonly used strains of E. coli without need for prior genetic manipulation of the host genome.
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KEYWORD
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Escherichia coli, recombinant protein expression, dps promoter, protein solubility
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